Dynamic analysis of Ca2+level during bovine oocytes maturation and early embryonic development
نویسندگان
چکیده
منابع مشابه
Dynamic analysis of Ca2+ level during bovine oocytes maturation and early embryonic development
Mammalian oocyte maturation and early embryo development processes are Ca(2+)-dependent. In this study, we used confocal microscopy to investigate the distribution pattern of Ca(2+) and its dynamic changes in the processes of bovine oocytes maturation, in vitro fertilization (IVF), parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT) embryo development. During the germinal v...
متن کاملDynamic Pattern of HOXB9 Protein Localization during Oocyte Maturation and Early Embryonic Development in Mammals
BACKGROUND We previously showed that the homeodomain transcription factor HOXB9 is expressed in mammalian oocytes and early embryos. However, a systematic and exhaustive study of the localization of the HOXB9 protein, and HOX proteins in general, during mammalian early embryonic development has so far never been performed. RESULTS The distribution of HOXB9 proteins in oocytes and the early em...
متن کاملpenicillin-streptomycin in the culture medium during in vitro maturation of bovine oocytes affects nuclear maturation and subsequent embryo development
introduction: standard concentrations of antibiotics in culture media are thought to have no detectable toxic effects on the cultured cells. since antibiotics are biologically active substances, the possibility that they interfere to some extent with cellular processes occurring in the cultured cells can not always be totally excluded. this study, therefore, was conducted to assess whether the ...
متن کاملeffect of various concentrations of glucose on bovine cumulus-oocyte complexes maturation, fertilization and early embryonic development in vitro
background & objectives : different energy substrates and nutrients can greatly influence oocyte nuclear and cytoplasmic maturation. effect of glucose on in-vitro oocyte maturation and subsequent early embryonic development is controversial. the objective of the present study was to verify the effect of various concentrations of glucose on cumulus-oocyte complexes maturation, fertilization and ...
متن کاملP-131: Effects of Heat Shock during Early Stage of Oocyte Maturation on Meiotic Progression and Subsequent Embryonic Development and Gene Expressionin Ovine
Background: Heat shock may affect different aspects of oocyte maturation and its subsequent development to the blastocyst stage. A series of in vitro experiments was performed to determine whether physiologically heat shock (41°C) disrupts the progression of the ovine oocytes through meiosis, activation and blastocyst formation. Materials and Methods: The cumulus-oocyte complexes (COCs) were as...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Veterinary Science
سال: 2011
ISSN: 1229-845X
DOI: 10.4142/jvs.2011.12.2.133